Composite

Part:BBa_K5286005:Design

Designed by: Mara Valverde Rascón   Group: iGEM24_TUM-Straubing   (2024-10-01)


CAHS106094+His Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 699
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 690
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 699
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 699
    Illegal NgoMIV site found at 707
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The basic BBa_K5286000 coding sequence part (CAHS94063 from Hypsibius dujardini) was assembled to the pET-16b vector without scars via Gibson Assembly.


Source

This part was assembled by the 2024 iGEM team from TUM campus Straubing. The coding sequence part (CAHS94063 from Hypsibius dujardini) was assembled into the pET-16b vector, which includes the HisTag.

References