Composite
Part:BBa_K5286005:Design
Designed by: Mara Valverde Rascón Group: iGEM24_TUM-Straubing (2024-10-01)
CAHS106094+His Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 699
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 690
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 699
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 699
Illegal NgoMIV site found at 707
Illegal AgeI site found at 743 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The basic BBa_K5286000 coding sequence part (CAHS94063 from Hypsibius dujardini) was assembled to the pET-16b vector without scars via Gibson Assembly.
Source
This part was assembled by the 2024 iGEM team from TUM campus Straubing. The coding sequence part (CAHS94063 from Hypsibius dujardini) was assembled into the pET-16b vector, which includes the HisTag.